Metabolic Pathways

Human and Mouse

Quantify the expression of hundreds of genes involved in core metabolic processes and signaling pathways in the context of cancer and immunity. The underlying molecular mechanisms behind alterations in metabolic pathways, signaling pathways, and cell stress can now be fully elucidated, giving researchers a complementary tool to traditional metabolite assays for profiling metabolic checkpoints and discovering potential therapeutic targets.

Benefits

01

Targeted applications include cancer metabolism, immunometabolism, and metabolic disease.

02

Profile 768 genes in human or mouse across 34 annotated pathways involved in 5 important themes for metabolic research.

03

Quantify the presence and relative abundance of 14 different immune cell types for immunometabolism studies.

04

Understand mechanisms of metabolic adaptation, metabolic switching and metabolic alterations, study changes in mitochondrial respiration and glycolysis, and advance efforts towards novel therapeutic targets.

Details & data

Key applications include:

Cancer Metabolism

  • How does cancer impact normal metabolic function?
  • Study key pathways involved in reprogrammed metabolism
  • Identify key changes that can be exploited for therapeutic development

Immunometabolism

  • How does the immune response change as a result of the alterations in metabolism?
  • Profile immune cell types
  • Link pathway activity to immune phenotype

Metabolic Disease

  • How is metabolism altered in disease?
  • Understanding disease pathogenesis
  • Identify potential therapeutic targets

Gene Coverage across Core Themes of Metabolism

Theme
Description
Pathways
# Human Genes# Mouse Genes
Biosynthesis and Anabolic PathwaysThe processes involved in the production of complex macromolecules by enzymecatalyzed biosynthetic pathways. The products of these pathways are required for nearly all cellular functions, including proliferation.Amino Acid Synthesis, Arginine Metabolism, Cell Cycle, Fatty Acid Synthesis, Glutamine Metabolism, Glycolysis, IDH1/2 Activity, Mitochondrial Respiration, Nucleotide Synthesis, Pentose Phosphate Pathway, Tryptophan/Kynurenine Metabolism, Vitamin and Cofactor Metabolism354348
Cell StressCells are impacted by the availability of nutrients and presence of toxic compounds. Adaptive responses to the stress are required for tumorigenesis, metastasis, and immune responses.DNA Damage Repair, Hypoxia, KEAP1/NRF2 Pathway, Reactive Oxygen Response8282
Nutrient Capture and Catabolic PathwaysThe processes involved in the breakdown of macromolecules, scavenging of cellular materials, or import of nutrients in order to stimulate ATP production or fuel anabolic pathways.Amino Acid Transporters, Autophagy, Endocytosis, Fatty Acid Oxidation, Glucose Transport, Lysosomal Degradation, Nucleotide Salvage161159
Metabolic SignalingThe pathways that are commonly disrupted in cancer cells or altered in immune cells that impact metabolic function. In the context of cancer, mutations allow these regulated signaling pathways to allow for metabolic change enabling tumorigenesis.AMPK, mTOR, MAPK, Myc, NF-kB, p53 Pathway, PI3K, TCR and Costimulatory Signaling, TLR Signaling237235
Transcriptional RegulationProcesses involved in the alteration of epigenetic and transcriptional activity of the cell that enables sustained metabolic reprogramming. This reprogramming allows for tumorigenesis and underlies stable changes in immune cell phenotype.Epigenetic Regulation, Transcriptional Regulation7769

Metabolic Pathways Panel Functional Annotations

Functional annotations for different pathways and processes were assigned to the genes in the Metabolic Pathways Panel. The pathways and processes that are included in this panel provide a comprehensive view of cellular metabolism.

Annotation
# Human
Genes
# Mouse
Genes
Annotation
# Human
Genes
# Mouse
Genes
AMPK4848mTOR5757
Amino Acid Synthesis9389MAPK5252
Amino Acid Transporters99Mitochondrial Respiration7372
Arginine Metabolism1616Myc2020
Autophagy5454NF-kB2727
Cell Cycle6565Nucleotide Salvage2323
DNA Damage Repair3131Nucleotide Synthesis4848
Endocytosis3941P53 Pathway3838
Epigenetic Regulation2626PI3K8282
Fatty Acid Oxidation2421Pentose Phosphate Pathway1919
Fatty Acid Synthesis1111Reactive Oxygen Response3737
Glucose Transport65TCR and Costimulatory Signaling5454
Glutamine Metabolism3130TLR Signaling3331
Glycolysis4037Transcriptional Regulation5951
Hypoxia1515Tryptophan/Kynurenine Metabolism3232
IDH1/2 Activity1515Vitamin and Cofactor Metabolism2828
KEAP1/NRF2 Pathway55Internal Reference2020
Lysosomal Degradation1616

Immune Cell Profiling

Genes included in the Human Metabolic Pathways Panel provide unique cell profiling data to measure the relative abundance of 14 different human immune cell types¹. The table below summarizes each cell type represented by gene content in the panel, as qualified through biostatistical approaches and selected literature in the field of immunology.

Cell Type
# Human
Genes
Cell Type
# Human
Genes
B cells9Mast Cclls4
CD451Neutrophils6
CD8 T cells2NK CD56dim cells3
Cytotoxic cells10NK cells2
Dendritic cells3T cells6
Exhausted CD84Th1 cells1
Macrophages4Treg1

Selected Panel References

  1. Danaher, P et al. Gene Expression Markers of Tumor Infiltrating Leukocytes. J Immunother Cancer. 2017;21(5):18.
  2. Peng, X et al. Molecular Characterization and Clinical Relevance of Metabolic Expression Subtypes in Human Cancers. Cell Reports. 2018;23(1):255-69.
  3. DeBerardinis RJ, Chandel, NS. Fundamentals of Cancer Metabolism. Science Advances. 2016;2(5).
  4. O’Neill LA et al. A Guide to Immunometabolism for Immunologists. Nature Reviews Immunology. 2016;16(9):553-65.
  5. Stine, ZE et al. MYC, Metabolism, and Cancer. Cancer Discovery. 2015;10:1024-39.
  6. Renner, K et al. Metabolic Hallmarks of Tumor and Immune Cells in the Tumor Microenvironment. Frontiers in Immunology. 2017;8:248.
  7. Andrejava, G and Rathmell, JC. Similarities and Distinctions of Cancer and Immune Metabolism in Inflammation and Tumors. Cell Metabolism. 2017;26(1):49-70.

Genes in panel

Pricing

Metabolic Pathways

$4,490.00

$
Step 3

Price

Number of samples
$4,490.00*
*price includes reagents
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