VistaPlex™ Assay Kits
Streamline development for your CellScape™ Precise Spatial Multiplexing experiments with validated, modular antibody panels and optimized protocols. Combine the assay kits together or add on your own biomarkers to conveniently investigate your sample with the right biomarkers for your research needs.
Accelerate Your CellScape Workflow with Confidence
Efficiently generate reliable multiplexed spatial data on the CellScape platform by avoiding time consuming antibody optimization and validation with VistaPlex Assay Kits.
Fluorescently labeled antibody panels targeting the most relevant immunophenotyping markers are evaluated using our robust validation procedure to ensure reproducible performance.
Versatile: Choose a kit for your sample type and cell profiling needs and add your unique biomarkers from our list or your own.
Efficient: Acquire results efficiently with a validated antibody panel, optimized staining plan, and straightforward protocol.
Reliable: Trust your results thanks to our validation process that uses multiple sample types and replicates.
Modular: Expand the plex of your assay by adding modules to the base Spatial Immune Profiling Assay Kit.
VistaPlex Assay Kits include:
Pre-validated fluorescently conjugated antibodies
Reagents and buffers
Optimized protocols
Browse Our Ready-to-Use VistaPlex Assay Kits
VistaPlex Assay Kits are available for a broad range of sample types including human FFPE, human fresh frozen (FF), and human PBMC. Choose the best kit to fit your needs.
| VistaPlex™ Assay Kits | Sample Type | Size | Product # |
|---|---|---|---|
| NEW: Tissue Architecture Assay Kit | Human FFPE | 10 samples | AbKT-3002-10RXN |
| NEW: Human Fresh Frozen Immune Profiling Assay Kit | Human FF | 10 samples | AbKT-2001-10RXN |
| Spatial Immune Profiling Assay Kit | Human FFPE | 10 samples | AbKT-3001-10RXN |
| T-Cell Assay Kit | Human PBMC | 10 samples | AbKT-1003-10RXN |
| Lymphoid and Myeloid Profiling Assay Kit | Human PBMC | 10 samples | AbKT-1001-10RXN |
| Broad Immune Profiling Assay Kit | Human PBMC | 10 samples | AbKT-1002-10RXN |
| Rapid Immune Profiling Assay Kit | Human PBMC | 10 samples | AbKT-1004-10RXN |
NEW: Tissue Architecture Kit
The Tissue Architecture Kit targets key epithelial, lymphatic, lamina, vasculature and connective tissue biomarkers and can be expanded by pairing with additional VistaPlex assay kits This 10-plex kit contains antibodies for:
CD138, Podoplanin, Vimentin, SMA, Beta-catenin, MUC1, CD31, CD34, Collagen-IV, E-cadherin, nuclear counterstain
NEW: Human Fresh Frozen Immune Profiling Assay Kit
The Human Fresh Frozen (FF) Immune Profiling Assay Kit enables spatial phenotyping of key immune populations and epithelial cells from human FF, OCT-embedded samples. This 14-plex kit contains antibodies for:
HLA-DR, CD56, CD11c, CD8, CD123, CD14, CD20, CD3, CD4, CD27, CD45, CD45RA, pan-Cytokeratin, nuclear counterstain
Spatial Immune Profiling Kit
The Spatial Immune Profiling Kit enables quantification of 14 phenotypic biomarkers that define key immune cell populations and subpopulations in human FFPE samples. The Spatial Immune Profiling is optimized for ease of use and throughput. This 14-plex assay kit consists of the following markers:
CD3, CD27, Ki-67, CD45, CD4, CD45RA, FoxP3, CD68, EpCAM, PD-1, CD8, PD-L1, Granzyme B, Nuclear counterstains
T Cell Assay Kit
The T-Ce ll Assay kit enables quantification of 8 phenotypic biomarkers that define T-cell populations and subpopulations i n human PMBC samples. The T Ce ll Assay i s optimized for ease of use and throughput. This 8-plex assay kit consists of the following markers:
Lymphoid and Myeloid Profiling Assay Kit
The Lymphoid and Myeloid Profiling Assay kit enables quantification of 12 phenotypic biomarkers that define T Cell, B Cell, Leukocyte, NK Cell, NKT Cell and Monocyte Cell populations and subpopulations in human PMBC samples. The Lymphoid and Myeloid Profiling Assay Kit is optimized for ease of use and throughput. This 12-plex assay kit consists of the following markers:
CD3, CD25, CD39, CD8, CD4, CD27, CD45RA, CD45, CD56, CD16, CD14, CD19
Broad Immune Profiling Assay Kit
The Broad Immune Profiling Assay kit enables quantification of 16 phenotypic biomarkers that define T Cell, B Cell, Leukocyte, NK Cell, NKT Cell, Monocyte, Dendritic and Exhaustion Cell populations and subpopulations in human PMBC samples. The Broad Immune Profiling Assay Kit is optimized for ease of use and throughput. This 16-plex assay kit consists of the following markers:
CD3, CD25, CD39, CD8, CD4, CD27, CD45RA, CD45, CD56, CD16, CD14, CD19, CD279, CD11c, HLA-DR, CD123
Rapid Immune Profiling Assay Kit
The Rapid Immune Profiling Assay kit enables quantification of 10 phenotypic biomarkers that define T Cell, B Cell, Leukocyte, NK Cell, NKT Cell, Monocyte and Dendritic Cell populations and subpopulations in human PMBC samples. The Rapid Immune Profiling Assay Kit is optimized for ease of use and throughput. This 12-plex assay kit consists of the following markers:
HLA-DR, CD56, CD11c, CD4, CD8, CD123, CD14, CD20, CD45, CD3
Ready to Accelerate Your CellScape Journey?
Jump start your next spatial biology project with the efficient and reliable antibody panels and protocols of VistaPlex Multiplex Assay Kits.
Validation Ensures Reliable Performance
Each VistaPlex Multiplex Assay Kit is extensively validated to ensure reliable performance. Learn more about our antibody validation process.
Antibody Selection
Each antibody clone is evaluated for target specificity, sensitivity, and reproducibility. Selection of suitable antibodies requires:
- Binding of intended target
- Differentiation of signal from background noise
- Detection of low levels of antigen
- Consistent results from similar samples
Antibody Conjugate Validation
After selection of suitable antibody clones, antibody conjugates are evaluated for proper expression and localization:
- Signal is specific
- Counter stains validate specificity
- Staining is distinct
- Localization and cellular distribution are confirmed
Multiplex Optimization
Multiplexed panels of antibody clones are optimized to minimize the number of cycles needed. All antibodies in the final multiplex panel must show:
- Distinct staining similar to single-plex
- No steric hindrance or nonspecific cross-talk
- Localization, cellular distribution, and percentage of target populations consistent with single-plex
Robust Reproducibility
Each tissue or cell sample used for validation is prepared carefully to ensure sample integrity. Multiplexing panels must pass all antibody validation criteria in:
- At least three suitable tissue types
- Each tissue tested with at least two replicates