Resource Center

Brochure

Canopy Spatial Services Brochure

Webinar

Immune Dysregulation in Inflammatory Bowel Disease

Jiangwei Zhang, PhD Senior Principal Scientist, Bristol Myers Squibb & J. Spencer Schwarz, Product Manager, Canopy Biosciences

Webinar

Spatial Immunome of Distal Tissues During Intestinal Inflammation

Gustavo Monasterio, DDS, PhD, Postdoctoral Fellow, Villablanca Lab, Karolinska Institute

Publication

T cell-derived interleukin-22 drives the expression of CD155 by cancer cells to suppress NK cell function and promote metastasis

Daria Briukhovetska, Javier Suarez-Gosalvez, Cornelia Voigt, Anamarija Markota, Anastasios D. Giannou, Maryam Schübel, Jakob Jobst, Tao Zhang, Janina Dörr, Florian Märkl, Lina Majed, Philipp Jie Müller, Peter May, Adrian Gottschlich, Nicholas Tokarew, Jöran Lücke, Arman Oner, Melanie Schwerdtfeger, David Andreu-Sanz, Ruth Grünmeier, Matthias Seifert, Stefanos Michaelides, Michael Hristov, Lars M. König, Bruno Loureiro Cadilha, Oleg Mikhaylov, Hans-Joachim Anders, Simon Rothenfusser, Richard A. Flavell, Daniela Cerezo-Wallis, Cristina Tejedo, María S. Soengas, Tobias Bald, Samuel Huber, Stefan Endres, and Sebastian Kobold

Publication

Bispecific antibodies redirect synthetic agonistic receptor modified T cells against melanoma

Florian Märkl1, Mohamed-Reda Benmebarek, Julius Keyl1, Bruno L Cadilha, Martina Geiger, Clara Karches, Hannah Obeck, Melanie Schwerdtfeger, Stefanos Michaelides, Daria Briukhovetska, Sophia Stock, Jakob Jobst, Philipp Jie Müller, Lina Majed, Matthias Seifert, Anna-Kristina Klüver, Theo Lorenzini, Ruth Grünmeier, Moritz Thomas, Adrian Gottschlich, Richard Klaus, Carsten Marr, Michael von Bergwelt-Baildon, Simon Rothenfusser, Mitchell P Levesque, Markus Vincent Heppt, Stefan Endres, Christian Klein and Sebastian Kobold

Publication

CagA-specific Gastric CD8+ Tissue-Resident T Cells Control Helicobacter pylori During the Early Infection Phase

Maximilian R A Koch, Ruolan Gong, Verena Friedrich, Veronika Engelsberger, Lorenz Kretschmer, Andreas Wanisch, Sebastian Jarosch, Anna Ralser Bob Lugen, Michael Quante, Michael Vieth, Riccardo Vasapolli, Christian Schulz, Veit R Buchholz, Dirk H Busch, Raquel Mejías-Luque, Markus Gerhard

Publication

Multimodal immune cell phenotyping in GI biopsies reveals microbiome-related T cell modulations in human GvHD

Sebastian Jarosch, Jan Ko¨hlen, Sakhila Ghimire, ..., Elvira D’Ippolito, Ernst Holler, Dirk H. Busch

Poster/Presentation

Precise Spatial Immune Profiling of PBMC Populations Using a VistaPlex™ ChipCytometry™ Assay Kit

Poster/Presentation

Spatial Immunoprofiling of Active Celiac Disease Tissue with ChipCytometry™

Product Bulletin

ChipCytometry™ Pre-Validated Antibody List – All Sample Types

Product Bulletin

CellScape™ Chip Kits for ChipCytometry™ Assays

Webinar

Immunohistochemistry and Spatial Biology: Using IHC for Next Gen Techniques

Christian Nievera, Ph.D., Canopy Biosciences

Poster/Presentation

Precise Spatial Multiplexing of Protein Biomarkers for Immune Profiling in Tissue Samples with ChipCytometry™

Webinar

Efficient and Flexible Spatial Multiplexing: Assay Development Guidelines for ChipCytometry™

Karen Kwarta, Canopy Biosciences

Publication

Microbeam Radiation Therapy Controls Local Growth of Radioresistant Melanoma and Treats Out-of-Field Locoregional Metastasis

Verdiana Trappetti, PhD, Marine Potez, PhD, Cristian Fernandez-Palomo, PhD, Vladislav Volarevic, Nahoko Shintani, PhD, Paolo Pellicioli, PhD, Alexander Ernst, PhD, David Haberthur, PhD, Jennifer M. Fazzari, PhD, Michael Krisch, PhD, Jean A. Laissue, MD, Robin L. Anderson, PhD, Olga A. Martin, PhD, and Valentin G. Djonov, MD

Webinar

ChipCytometry™ Multiplexing: Practical, Approachable, Spatial Biology

J. Spencer Schwarz, Canopy Biosciences

Poster/Presentation

SITC 2022 – Precise spatial multiplexing in clinical breast tumor specimens with a 60-plex ChipCytometry assay

Case Study

Profiling the Tumor Microenvironment of Neuroblastoma using ChipCytometry™

Publication

Modeling intercellular communication in tissues using spatial graphs of cells

David S. Fischer, Anna C. Schaar & Fabian J. Theis

Webinar

Realizing the Potential of Spatial Biology for a Noncanonical Model

Shaye Hagler, Ph.D., Canopy Biosciences

Webinar

Validating Antibodies for Increasingly Sensitive Quantitative Applications

Caleb Sutton, Ph.D., Canopy Biosciences

Webinar

Technique Talk: Developing Spatial Multiomic Maps

Webinar

Investigating Immune Regulation by Small Extracellular Vesicles to Attenuate Chronic Pain Using High-Plex Spatial Imaging

Xuan Luo, Department of Pharmacology and Physiology, Drexel University College of Medicine

Product Bulletin

ChipCytometry™ ZellSafe™ Chip Kits

Product Bulletin

ChipCytometry™ CellScape™ Chip Kits

Webinar

Generating Actionable Spatial Biology Data with CellScape™ Precise Spatial Multiplexing

J. Spencer Schwarz, Canopy Biosciences

Poster/Presentation

AGBT 2022 – Precise spatial multiplexing of immune cell diversity in clinical breast tumor specimens with ChipCytometry

Product Bulletin

ChipCytometry™ T Cell Assay Kit

Product Bulletin

ChipCytometry™ Spatial Immune Profiling Assay Kit

Webinar

Fast Track to Precise Spatial Multiplexing: ChipCytometry™ Assay Kits and Assay Development Guidelines

Karen Kwarta, Ph.D., Canopy Biosciences

Webinar

Novel Opportunities to Extend the Clinical Potential of Microbeam Radiotherapy

Olga Martin, Ph.D., Institute of Anatomy, University of Bern

Publication

ChipCytometry™ for multiplexed detection of protein and mRNA markers on human FFPE tissue samples

Sebastian Jarosch, Jan Köhlen, Sabrina Wagner, Elvira D’Ippolito, and Dirk H. Busch

Poster/Presentation

Immunology 2022 – Precise Spatial Multiplexing of Immune Cell Diversity in Clinical Tumor Samples with ChipCytometry™

Product Bulletin

ChipCytometry™ Pre-Validated Antibody List – Non-Human Primate Samples

Product Bulletin

ChipCytometry™ Pre-Validated Antibody List – Human Cell Suspension

Product Bulletin

ChipCytometry™ Pre-Validated Antibody List – Human Tissue

Product Bulletin

ChipCytometry™ Pre-Validated Antibody List – Mouse Cell Suspension

Product Bulletin

ChipCytometry™ Pre-Validated Antibody List – Mouse Tissue

Poster/Presentation

SITC 2021 – Precise Spatial Multiplexing for Immune Profiling in Non Small Cell Lung Cancer FFPE Samples with ChipCytometry™

Poster/Presentation

CYTO 2021 – Precise Spatial Multiplexing of Immune Populations in Human Colorectal Cancer FFPE Samples with ChipCytometry™

Webinar

Spatial Biology in Physiology and Pathology

Elana Fertig, Ph.D., Johns Hopkins University | Sanjar Srivatsan, Ph.D., University of Washington

Webinar

Capturing Heterogeneity: How Single Cell Analysis Reshapes Health and Disease Research

Timothy O'Sullivan, Ph.D, University of California, Los Angeles | Jacob Blum, Stanford University

Webinar

Human immune cell compartments in the spleen of humanized mice

Prof. Christian Münz, Institute of Experimental Immunology, University of Zürich

Webinar

Analysis of T cell subsets in human tissue using ChipCytometry™

Prof. Paul Klenerman, Oxford University

Webinar

The Influence of Rare Cells in the Nervous System

Sebastian Lewandowski, Ph.D, Karolinska University | Philip Hasel, Ph.D., NYU School of Medicine

Webinar

High-plex immune profiling of clinical PBMCs using ChipCytometry™ – Addressing challenges with clinical sample logistics

Tim Sindelar, M.S., Canopy Biosciences

Poster/Presentation

AACR 2022 – Precise Spatial Multiplexing for Immune Profiling in FFPE Tissues with ChipCytometry™

Webinar

Precise Spatial Multiplexing for Quantitative Single-Cell Phenotyping in Tissues

Thomas Campbell, Ph.D., Canopy Biosciences

Webinar

Seeing is Believing: The Power of ChipCytometry™ Multiplexing

J. Spencer Schwarz, M.S., CanopyBiosciences

Webinar

Multiplexed Imaging & Automated Signal Quantification

Sebastian Jarosch, M.Sc., Institute for Immunology, Technical University of Munich (TUM)

Brochure

CellScape Brochure

Webinar

Spatial Fest 2022: Exploring the Landscape with ChipCytometry™

Thomas Campbell, Ph.D, Canopy Biosciences | Sebastian Jarosch, M.Sc., Technical University of Munich (TUM) | Sarah Harris, Bristol Myers Squibb | Rachel Maddux, Ph.D, Bristol Myers Squibb | J. Spencer Schwarz, M.S., Canopy Biosciences

App Note/Tech Note

Optimizing Collection of Clinical Blood Samples for ChipCytometry™

Publication

Stimulatory MAIT cell antigens reach the circulation and are efficiently metabolised and presented by human liver cells

Martin J. Lett, Hema Mehta, Adrian Keogh, Tina Jaeger, Maxime Jacquet, Kate Powell, Marie-Anne Meier, Isabel Fofana, Hassan Melhem, Jürg Vosbeck, Gieri Cathomas, Andres Heigl, Markus H. Heim, Emanuel Burri, Kirsten D. Mertz, Jan Hendrik Niess, Otto Kollmar, Christoph J. Zech, Robert Ivanek, Urs Duthaler, Paul Klenerman, Deborah Stroka, Magdalena Filipowicz Sinnreich

App Note/Tech Note

Antibody Validation for ChipCytometry™

Publication

Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry™

Sebastian Jarosch, Jan Köhlen, Rim S.J. Sarker, Katja Steiger, Klaus-Peter Janssen, Arne Christians, Christian Hennig, Ernst Holler, Elvira D'Ippolito, and Dirk H. Busch

Product Bulletin

ChipCytometry™ Assay Catalog

App Note/Tech Note

Rare Cell Discovery with ChipCytometry™

Poster

Novartis — ChipCytometry™ identifies the presence of uncommon B-cell subsets in inflamed tonsils associated with autoimmunity

Publication

Der p1 and Der p2-Specific T Cells Display a Th2, Th17, and Th2/Th17 Phenotype in Atopic Dermatitis

Lennart M Roesner, Annice Heratizadeh, Gabriele Begemann, Petra Kienlin, Susanne Hradetzky, Margarete Niebuhr, Britta Eiz-Vesper, Christian Hennig, Gesine Hansen, Véronique Baron-Bodo, Philippe Moingeon, Thomas Werfel

Webinar

Multiplex Proteomics for Immune Profiling

Publication

Human intestinal tissue-resident memory T cells comprise transcriptionally and functionally distinct subsets

Michael E.B. FitzPatrick, Nicholas M. Provine, Lucy C. Garner, ..., Elizabeth Soilleux, Paul Klenerman, Philip J. Allan

Poster

Fraunhofer ITEM — Cellular analysis of induced sputum by ChipCytometry

Publication

Differential expression patterns of glycosphingolipids and C-type lectin receptors on immune cells in absence of functional regulatory T cells

Adan C. Jirmo, Charlotte Rossdam, Ruth Grychto, Christine Happle, Rita Gerardy-Schahn, Falk F. R. Buettner, Gesine Hansen

Publication

Monocyte/macrophage lineage commitment and distribution are affected by the lack of regulatory T cells in scurfy mice

Jelena Skuljec, Maciej Cabanski, Ewa Surdziel, Nico Lachmann, Sebastian Brennig, Refik Pul, Adan C Jirmo, Anika Habener, Julia Visic, Kathleen Dalüge, Christian Hennig, Thomas Moritz, Christine Happle, Gesine Hansen

Webinar

Clinical ChipCytometry™ — Biobanking PBMCs for Protein Biomarker Analysis in Clinical Trials

Publication

In-Depth Immune-Oncology Studies of the Tumor Microenvironment in a Humanized Melanoma Mouse Model

Jonathan Schupp, Arne Christians, Niklas Zimmer, Lukas Gleue, Helmut Jonuleit, Mark Helm andAndrea Tuettenberg

Publication

Lung function and inflammation during murine Pseudomonas aeruginosa airway infection

Florian Wölbeling, Antje Munder, Tanja Kerber-Momot, Detlef Neumann, Christian Hennig, Gesine Hansen, Burkhard Tümmler, Ulrich Baumann

Poster

Merk — Detection of PD-L1 and PD-L2 on circulating tumor cells using ChipCytometry

Publication

A preliminary study for the assessment of PD-L1 and PD-L2 on circulating tumor cells by microfluidic-based chipcytometry

Jinkai Teo, Anja Mirenska, Meihui Tan, Yifang Lee, Janice Oh, Lewis Z Hong, Richard Wnek, Yoon-Sim Yap, Shian-Jiun Shih, Ali Asgar S Bhagat, Chih-Liang Chin & David AG Skibinski

Publication

Long-term in vivo microscopy of CAR T cell dynamics during eradication of CNS lymphoma in mice

Matthias Mulazzani, Simon P. Fräßle, Iven von Mücke-Heim, Sigrid Langer, Xiaolan Zhou, Hellen Ishikawa-Ankerhold, Justin Leube, Wenlong Zhang, Sarah Dötsch, Mortimer Svec, Martina Rudelius, Martin Dreyling, Michael von Bergwelt-Baildon, Andreas Straube, Veit R. Buchholz, Dirk H. Busch, and Louisa von Baumgarten

Publication

Successful treatment of autoimmune and lymphoproliferative complications of patients with intrinsic B-cell immunodeficiencies with Rituximab

Christian Hennig, Ulrich Baumann, Claudia Ilginus, Gerd Horneff, Juergen Foell, Gesine Hansen

Poster

AstraZeneca/MedImmune — Comparison of human whole blood FC to ChipCytometry™

Publication

Immunophenotyping of cerebrospinal fluid cells by Chipcytometry™

Martin W. Hümmert, Sascha Alvermann, Stefan Gingele, Catharina C. Gross, Heinz Wiendl, Anja Mirenska, Christian Hennig & Martin Stangel

Poster

Takeda — High-parameter profiling of psoriatic tissue

Publication

Pulmonary transplantation of macrophage progenitors as effective and long-lasting therapy for hereditary pulmonary alveolar proteinosis

Christine Happle, Nico Lachmann, Jelena Škuljec, Martin Wetzke, Mania Ackermann, Sebastian Brennig, Adele Mucci, Adan Chari Jirmo, Stephanie Groos, Anja Mirenska, Christina Hennig, Thomas Rodt, Jens P. Bankstahl, Nicolaus Schwerk, Thomas Moritz, and Gesine Hansen

Publication

Quantitative analysis of endotoxin-induced inflammation in human lung cells by Chipcytometry™

Saskia Carstensen, Olaf Holz, Jens M. Hohlfeld, Meike Müller

Webinar

Three Challenges with Highly Multiplexed Spatial Proteomics in Clinical Tissue Samples

J. Spencer Schwarz, M.S., Thomas Campbell, Ph.D., CanopyBiosciences

Publication

Gain-of-function STAT1 mutations are associated with intracranial aneurysms

Mete Dadak, Roland Jacobs, Jelena Skuljec, Adan Chari Jirmo, Özlem Yildiz, Frank Donnerstag, Niklas Thomas Baerlecken, Reinhold Ernst Schmidt, Heinrich Lanfermann, Thomas Skripuletz, Philipp Schwenkenbecher, Christoph Kleinschnitz, Hayrettin Tumani, Martin Stangel, Refik Pul

Publication

Defining T Cell Subsets in Human Tonsils using ChipCytometry™

Joachim P. Hagel, Kyle Bennett, Francesca Buffa, Paul Klenerman, Christian B. Willberg and Kate Powell

Publication

A versatile platform for comprehensive chip-based explorative cytometry

Christian Hennig, Nico Adams, Gesine Hansen

Poster

Pfizer — Cross-technology comparison of ChipCytometry vs Flow Cytometry for the measurement of T cell phenotypic and functional markers

Publication

Sjögren’s Syndrome Minor Salivary Gland CD4+ Memory T Cells Associate with Glandular Disease Features and Have a Germinal Center T Follicular Helper Transcriptional Profile

Michelle L. Joachims, Kerry M. Leehan, Mikhail G. Dozmorov, Constantin Georgescu, Zijian Pan, Christina Lawrence, M. Caleb Marlin, Susan Macwana, Astrid Rasmussen, Lida Radfar, David M. Lewis, Donald U. Stone, Kiely Grundahl, R. Hal Scofield, Christopher J. Lessard, Jonathan D. Wren, Linda F. Thompson, Joel M. Guthridge, Kathy L. Sivils, Jacen S. Moore, and A. Darise Farris

Publication

Identification and quantification of basophils in the airways of asthmatics following segmental allergen challenge

Dorothea Dijkstra, Christian Hennig, Gesine Hansen, Heike Biller, Norbert Krug, Jens M Hohlfeld

Publication

α-NAC-Specific Autoreactive CD8+ T Cells in Atopic Dermatitis Are of an Effector Memory Type and Secrete IL-4 and IFN-γ

Lennart M Roesner, Annice Heratizadeh, Susanne Wieschowski, Irene Mittermann, Rudolf Valenta, Britta Eiz-Vesper, Christian Hennig, Gesine Hansen, Christine S Falk, Thomas Werfel

Webinar

ChipCytometry™: Software Update Tutorial #2

Publication

TCR and Inflammatory Signals Tune Human MAIT Cells to Exert Specific Tissue Repair and Effector Functions

Tianqi Leng, Hossain Delowar Akther, Carl-Philipp Hackstein, Kate Powell, Thomas King, Matthias Friedrich, Zoe Christoforidou, Sarah McCuaig, Mastura Neyazi, Carolina V. Arancibia-Cárcamo, Joachim Hagel, Fiona Powrie, Oxford IBD Investigators, Raphael Sanches Peres, Val Millar, Daniel Ebner, Rajesh Lamichhane, James Ussher, Timothy S.C. Hinks, Emanuele Marchi, Chris Willberg, Paul Klenerman

Poster

Novartis — Long-term sample storage and re-interrogation in clinical trial support

Webinar

ChipCytometry™ — Analysis of High-plex Proteomics in Tissue Samples