Rapid Immune Profiling Assay Kit

10-Plex for Human PBMC Samples and ZellScannerONE™

VistaPlex™ Assay Kits for ChipCytometry offer a ready to use, reliable and customizable solution to fast-track precise spatial multiplexing.

Designed to provide a convenient, flexible, and modular analysis of immune cells, the Rapid Immune Profiling Assay kit enables quantification of 10 phenotypic biomarkers that define T Cell, B Cell, Leukocytes, NK Cell, NKT Cell, Monocyte and Dendritic Cell populations and sub-populations in human PMBC samples. Immune cells can be characterized in 2 short staining cycles.  Validation was performed on the ChipCytometry™ platform on 3 blood donors in duplicate, in addition to individual antibody validation. The Rapid Immune Profiling Assay Kit contains primary antibodies and antibody diluent and is optimized for ease of use and throughput.

PBMC samples showing 10 biomarkers quantified across immune cells.
PBMC samples showing 10 biomarkers quantified across immune cells. The biomarkers are used to phenotype immune cell populations such as those shown here with defining markers displayed.
Biomarkers identified and quantified:
Cell population characterized and quantified:
LeukocytesT CellsNK CellsNKT
T CytotoxicT HelperB CellsMonocytes
Dedritic CellsMyeloid DCsPlasmacytoid CDs

Details & Data

Gating Strategy

Hierarchical gating strategies define cell phenotypes. From image data, each cell is captured by automated segmentation, and the biomarker expression is quantified using HDR imaging to measure fluorescence intensity for every cell. Standard FCS files are generated to enable the identification of cell phenotypes via classic flow cytometry-like hierarchical gating.

Representative dot plots demonstrate a gating strategy to characterize and quantitate immune cells.
Cell PopulationParent GateGating Strategy
T cellsCD45+ LeukocytesCD45+ CD3+ CD56-
NK CellsCD45+ LeukocytesCD45+ CD3- CD456+
NKT CellsCD45+ LeukocytesCD45+ CD3+ CD56+
T CytotoxicT CellsCD45+ CD3+ CD56- CD4- CD8+
T HelperT CellsCD45+ CD3+ CD56- CD4+ CD8-
B CellsCD45+ LeukocytesCD45+ CD3- CD56- CD14- CD20+
MonocytesCD45+ LeukocytesCD45+ CD3- CD56- CD14+ CD20-
Dendritic CellCD45+ CD3- CD14- CD20-CD45+ CD3- CD14- CD20- HLA-DR+
Myeloid DCsDendritic CellsCD45+ CD3- CD14- CD20- HLA-DR+ CD11c+ CD123-
Plasmacytoid DCsDendritic CellsCD45+ CD3- CD14- CD20- HLA-DR+ CD11c- CD123+


The ChipCytometry™  platform captures biological variability, while maintaining technical reproducibility. For the Rapid Immune Profiling Assay Kit, technical replicates were tested on 3 different donors. Fresh PMBC samples were used to confirm intra-assay and inter-assay reproducibility. To ensure high quality data can be obtained on a variety of sample types, the assay kit was also successfully tested on cryopreserved samples.

Ready to Use and Customizable

The Rapid Immune Profiling Assay Kit has been optimized to maximize throughput by minimizing the number of cycles required for analysis. Additional cycles may be added for further customization of your high-plex assay

CycleAntibodyCorresponding Filter SetVol. AntibodyDiluent VolumeIncubation Time
1HLA-DRFSPerCP10μL250μL5 min
2CD123FSPerCP10μL250μL5 min

Rapid Immune Profiling Assay Kit Documentation

Download the Protocol for easy-to-use instructions for the Rapid Immune Profiling Assay kit. The Assay Template file can be imported onto the ZKW App to pre-load the assay instructions for quick and easy assay set up.

Store all kit contents at 4°C, protected from light.

Additional materials required: ZellSafe™ Chips (28050606/01-010) and Wash Buffer (28050606/07-004).


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