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Sample Submission Guide for Canopy Biosciences Services

Below are guidelines for submitting samples to Canopy Biosciences.  We’ve included several services that we offer.  We accept isolated RNA and  also offer RNA isolation for a fee. The guidelines listed here are recommendations; if your sample size and concentration is different, we are happy to discuss individual needs and experimental design.

All samples should be shipped to the following address:
Canopy Biosciences
Attn: Sample Receiving
4340 Duncan Ave., Suite 220
St. Louis, MO 63110

Please notify Canopy of your shipment and tracking information.  Send this information to Samples@canopybiosciences.com and include your project ID that has been assigned and an electronic copy of your sample key.

For all samples, we will contact you with any issues in RNA QC prior to run.

Domestic Shipments
Samples should be shipped overnight on dry ice unless otherwise noted below. Please place your samples in a cardboard box, place the box in a sealed plastic bag, and bury the plastic bag in dry ice. Your outer box should include a styrofoam insert. Please ensure your shipment will arrive on a weekday.

International Shipments
Samples should be shipped on dry ice unless otherwise noted below. We recommend using World Courier for international shipments. Please place your samples in a cardboard box, place the box in a sealed plastic bag, and bury the plastic bag in dry ice. Your outer box should include a styrofoam insert. Ensure that you have enough dry ice for the duration of the shipment; consult your shipping courier for specific guidelines if needed. Please ensure your shipment will arrive on a weekday.

Labeling
Tubes must be labeled clearly and match the sample key provided.  Any labeling schemes will be accepted, but we recommend using simplified nomenclature that blinds the laboratory from the experimental treatment conditions.  When possible, sample randomization should be performed prior to shipment.

Isolated mRNA
For NanoString, we request 400 ng of isolated RNA at a concentration of >20 ng/µL; for RNA Seq~1 µg at a concentration >50 ng/µL is requested for standard poly-A enrichment or rRNA depletion library prep methods.  For low input mRNA experiments, we require a minimum of 1 ng at >50 pg/µL. RNA should be DNase-treated and eluted in nuclease-free water.  Do not dilute samples based on Nanodrop readings as this is not an accurate assessment of RNA concentration.  Please provide preliminary sample concentration on the sample key.  RNA quantity and quality will be assessed by Qubit and Bioanalyzer analysis upon arrival.  Purified RNA should be stored at -80°C prior to shipment. Samples should be shipped on dry ice.

Tissue
Tissue should be flash-frozen or preserved in RNAlater solution according to the manufacturer’s instructions.  We recommend sending between 5-30 mg of tissue in a 1.5 mL microcentrifuge tube.  Please indicate the tissue type and estimated mass (mg) on the sample submission sheet.  Samples should be shipped on dry ice.

Blood
Blood sample should be collected in PAXgene Blood RNA Tubes according to the manufacturer’s instructions.  Please be sure to incubate freshly collected samples at room temperature for a minimum of 2 hours prior to transferring to the freezer (-20°C).  Tubes should be shipped on dry ice.

Serum/Plasma
500 µL of serum or plasma is recommended for NanoString services.  Samples should be stored at -80°C prior to shipment. Tubes should be shipped on dry ice.

For miRNA assays, blood should be collected in EDTA (K2 or K3) tubes.  Plasma should be promptly collected and frozen at -80°C in 500 µL aliquots.

Cell Pellets
Cell numbers between 150,000 and 1M are preferred.  Cell pellets can be resuspended in 350 µL of Qiagen buffer RLT.  Cell pellets should be stored at -80°C prior to shipment.  Please indicate the number of submitted cells on the sample submission form.  Samples should be shipped on dry ice.

FFPE
Send 3 x 10 µm FFPE scrolls in a standard 1.5 mL microcentrifuge tube.  Please ship samples with ice packs.  DO NOT ship on dry ice.

RNA isolation services available; $900 for 12 samples

For questions regarding sample types not listed here please contact Samples@canopybiosciences.com

 

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