Fibrosis

Human and Mouse

Uncover the mechanisms of disease pathogenesis, identify biomarkers of progression, and develop signatures for therapeutic response with the nCounter Fibrosis Panel. Profile 770 genes across 51 annotated pathways involved in the 4 stages of fibrosis: initiation, inflammation, proliferation, and modification. This gene expression panel combines hundreds of genes involved in the initial tissue damage response, chronic inflammation, proliferation of pro-fibrotic cells, and tissue modification that leads to fibrotic disease of the lungs, heart, liver, kidney, and skin.

Benefits

01

Study Pathogenesis and identify biomarkers for fibrotic diseases of the lungs, heart, liver, kidney and skin.

02

Quantify the relative abundance of 14 different immune cell types.

03

Understand signaling cascades from cell stress to inflammation.

04

Identify biomarkers for therapeutic response.

Details & data

Key applications include:

Lung Disease Research
  • Idiopathic pulmonary fibrosis
  • Cystic fibrosis
Liver Damage Research
  • Cirrhosis
  • Chronic hepatitis C infection
  • Non-alcoholic steatohepatitis (NASH)
  • Alcoholic liver disease
Kidney Disease Research
  • Renal fibrosis
Skin Disease Research
  • Scleroderma
  • Nephrogenic fibrosing dermopathy
  • Scleromyxedma
  • Scleredema
  • Eosinophilic fasciitis
Heart Disease Research
  • Cardiomyopathy
  • Ischemic heart disease

Gene Coverage Across the Four Stages of Fibrosis

StageDescriptionPathways# Human
Genes
# Mouse
Genes
InitiationCell and tissue damage, often specific to an organ or fibrotic disease, initiates a cascade of stress and immune responses.
Autophagy, Cholesterol Metabolism, Cytosolic DNA Sensing, De Novo Lipogenesis, Endotoxin Response, Fatty Acid Metabolism, Gluconeogenesis, Insulin Resistance/Signaling, MAPK Cell Stress, mTOR, Oxidative Stress, PPAR Signaling, Proteotoxic Stress, SASP369369
InflammationInflammation is one of many responses to the initial damage, involving multiple immune cell types and signaling pathways. Chronic inflammation drives the proliferation of pro-fibrotic cells and tissue modification.
Adenosine Pathway, Chemokine Signaling, Complement Activation, Cytokine Signaling, Granulocyte Activity, Inflammasome, M1/M2 Activation, MHC Class II Antigen Presentation, Neutrophil Degranulation, NF-kB, Phagocytic Cell Function, Platelet Degranulation, Th1/ Th2/Th17 Differentiation, TLR Signaling, Type I/Type II Interferon297297
ProliferationDifferentiation and proliferation of myofibroblasts are driven by upstream inflammation. These cells drive the wound-healing response that results in fibrotic damage.Cell Cycle, ECM Synthesis, EMT, Focal Adhesion Kinase, Hedgehog Signaling, Hypoxia, Myofibroblast Regulation, Notch, PDGF Signaling, PI3K-Akt, TgfBeta, Wnt
159161
ModificationImmune and myofibroblast cells contribute to extracellular matrix modification and tissue alterations that are characteristic of fibrotic disease.Angiogenesis, Apoptosis, Collagen Biosynthesis & Modification, ECM Degradation, Epigenetic Modification, Hippo Pathway, Regulated Necrosis
287287

Fibrosis Panel Functional Annotations

Functional annotations for different pathways and processes were assigned to the genes in the Fibrosis Panel. The pathways and processes that are included in this panel provide a comprehensive view of the pathogenesis of fibrotic disease.

Annotation# Human Genes
# Mouse Genes
Annotation# Human Genes
# Mouse Genes
Adenosine Pathway5153M1 Activation1212
Angiogenesis3737M2 Activation1212
Autophagy3939MAPK Cell Stress6767
Cell Cycle4949MHC Class II Antigen Presentation1615
Chemokine Signaling2322mTOR3636
Cholesterol Metabolism2624Myofibroblast Regulation2525
Collagen Biosynthesis & Modification2727Neutrophil Degranulation6773
Complement Activation2219NF-kB4140
Cytokine Signaling6058Notch2020
De Novo Lipogenesis2020Oxidative Stress1818
ECM Degradation4244PDGF Signaling2929
ECM Synthesis3838Phagocytic Cell Function2727
EMT8888PI3K-Akt8989
Endotoxin Response4241Platelet Degranulation3334
Epigenetic Modification2424PPAR Signaling2833
Fatty Acid Metabolism5557Programmed Cell Death4141
Focal Adhesion Kinase4949Proteotoxic Stress4343
Glucogenesis2019SASP1515
Granulocyte Activity3434TGF-beta3838
Hedgehog Signaling2424Th1 Differentiation1313
Hippo Pathway1313Th17 Differentiation2424
Hypoxia1616Th2 Differentiation1313
Inflammasome3232TLR Signaling6362
Insulin Resistance3838Type I Interferon2431
Insulin Signaling1616Type II Interferon3037
Internal Reference Genes1010Wnt3838

Immune Cell Profiling

Genes included in the Human Fibrosis Panel provide unique cell profiling data to measure the relative abundance of 14 different human immune cell types . The table below summarizes each cell type represented by gene content in the panel, as qualified through biostatistical approaches and selected literature in the field of immunology.

Cell Type
Associated Human Genes
Cell Type
Associated Human Genes
B cellsBLK, CD19, MS4A1, TNFRSF17, FCRL2, KIAA0125/FAM30A, PNOC, SPIB, TCL1AMast Cells4MS4A2, TPSAB1, CPA3, HDC, TPSB2
CD45CD45NeutrophilsCSF3R, S100A12, CEACAM3, FCAR, FCGR3A, FCGR3B, FPR1, SIGLEC5
CD8 T cellsCD8A, CD8BNK CD56dim cellsIL21R, KIR2DL3, KIR3DL1, KIR3DL2
Cytotoxic cellsCTSW, GNLY, GZMA, GZMB, GZMH, KLRB1, KLRD1, KLRK1, PRF1, NKG7NK cellsNCR1, XCL2, XCL1
Dendritic cellsCCL13, CD209, HSD11B1T cellsCD3D, CD3E, CD3G, CD6, SH2D1A, TRAT1
Exhausted CD8CD244, EOMES, LAG3, PTGER4Th1 cellsTBX21
MacrophagesCD163, CD68, CD84, MS4A4ATregFOXP3

Genes in panel

Pricing

Fibrosis

$4,990.00

$
Step 3

Price

Number of samples
$4,990.00*
*price includes reagents
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