miRNA CRISPR Knockout Kits
- All-in-one dual-gRNA CRISPR/Cas9 plasmids
- Cutting-edge bioinformatics algorithm for gRNA design
- Genome editing can be conveniently confirmed by genomic PCR
A single guide RNA only introduces minor genomic modifications, which has poor efficacy at knocking out miRNA gene functions. To address this challenge, Nawgen has developed a dual-gRNA construct system to target flanking genomic regions of the miRNA gene, leading to complete excision of the miRNA gene. The scheme of this strategy is shown in the figure above. This approach has many advantages: it allows for complete loss of function of the miRNA gene, provides a larger target area to select potent gRNAs, and genome editing can be conveniently confirmed by genomic PCR instead of the more laborious T7 endonuclease assay.
More Technical Information
Please click on the link below for more information about our dual-gRNA CRISPR system.
Dual-gRNA CRISPR system technical notes