All the power of CRISPR, in one simple kit.
CRISPR Complete kits from Canopy Biosciences contain everything you need to perform your CRISPR experiments, all provided in one simple box. Our CRISPR kits are tailor-made for your project by our team of genetic engineers, who have extensive experience editing cells and animal models.
CRISPR kits are available for almost any project type, including knockouts, knock-ins, TUNR knockdowns, point mutations, gene replacements, humanizations, tagging, and many more.
To get started, simply complete our short online ordering form, and we’ll send you a CRISPR kit with everything you need in as few as 5 days.
Included in the CRISPR Complete Kit:
- crRNA—custom-designed for your project
- tracrRNA
- Cas9 Nuclease Protein
- Donor construct—custom-designed for your project
(may be provided as oligonucleotide or plasmid, depending on your project needs)
ABOUT THE CRISPR-CAS9 SYSTEM
The CRISPR-Cas9 system relies on two components—a targeting domain (sgRNA–orange) and a nuclease domain (Cas9–blue). This system allows for the generation of double-stranded breaks, at highly specific and targeted sites within the genome. Harnessing the cell’s own natural repair mechanisms, we can cause frameshift mutations leading to gene knockout or provide a template for the cell to repair from leading to targeted insertions.
Image credit: Ding Y, Li H, Chen L-L and Xie K (2016) Recent Advances in Genome Editing Using CRISPR/Cas9. Front. Plant Sci. 7:703. doi: 10.3389/fpls.2016.00703
