More efficient gene editing with fewer off target effects.

Cas9 is the nuclease component of the
CRISPR/Cas9 system.

 

  • Isolated from S. pyogenes
  • Supplied at 10 µg/µl in a glycerol stock solution to avoid freeze/thaw related degradation
  • Protein format is more efficient than plasmid or RNA-based systems
  • 2 Nuclear localization signals (NLS) for increased nuclear transport and efficiency
  • Fewer off target effects than plasmid-delivered Cas9

More efficient than plasmid DNA Cas9

T7E1 assay demonstrating cutting efficiency at target and off target sites.

Adapted from Sojung Kim et al. Genome Res. 2014;24:1012-1019

Faster editing with Cas9
protein vs. plasmid

Cas9 protein is fully active within 24 hours of electroporation vs. 3 days with plasmid DNA.

Adapted from Sojung Kim et al. Genome Res. 2014;24:1012-1019

Faster degradation = fewer off target effects

Cas9 protein is fully degraded approximately 24 hours following electroporation vs. >3 days with plasmid DNA. Residual Cas9 expression has been proposed as an important source of off target effects.

Adapted from Sojung Kim et al. Genome Res. 2014;24:1012-1019