Canopy
Zellkraftwerk

CRISPR Cell Line Service

Have a project perfect for our CRISPR Complete Kit or the TUNR Flexible Gene Editing system but need a CRISPR cell line service to perform the editing? No problem–Canopy Biosciences offers a comprehensive custom CRISPR cell line service based on the CRISPR-Cas9 system. Just tell us your target gene and the mutation that you want to make, and with our CRISPR cell line service, we’ll make the knockout, knock-in, point mutation, or other modification in your favorite cell line.

Step 1: Fill out our Project Evaluation Form.

Canopy’s team of cell line engineers will evaluate the feasibility and scope of your project and provide a formal quotation

Fill out our project evaluation form here.

Step 2: Choose from one of our cell lines or ship us yours.

We’ll evaluate your cell line’s amenability for gene editing.

Step 3: Canopy will custom-design and validate reagents.

All reagents needed to perform your project, including CRISPR guide RNAs and donors for insertion projects, will be custom-designed for your project.

Step 4: Canopy will perform the gene editing.

Canopy will nucleofect your cells with the editing reagents, screen cell pools for modifications, single cell dilute, and screen and identify clones containing your desired mutation.

Step 5: Editing complete!

Following clonal expansion, Canopy will return you your cell line, tailored with your exact modifications.

Cell Lines Available

Canopy has experience editing >80 different cell lines, including popular lines such as HEK-293, HCT-116, NIH 3T3, K562, and HeLa, as well as ES cells and iPSC. Have a unique or highly specialized line? No problem—simply send your line to us and we will characterize the line to see if its amenable to gene editing.

Project Types Available

  • TUNR Insertion
  • Gene Knockout
  • Targeted Deletion
  • Codon Change
  • Targeted Integration
  • Point Mutation
  • Gene Tagging
  • . . . and more

Pricing

Knockout projects start at $12,900 and knock-ins at $19,900. Your project may vary based on complexity, fill out our form for a formal project evaluation and quotation.

Get your project started by filling out our form.

crispr diagram

ABOUT THE CRISPR-CAS9 SYSTEM

The CRISPR-Cas9 system relies on two components—a targeting domain (sgRNA–orange) and a nuclease domain (Cas9–blue). This system allows for the generation of double-stranded breaks, at highly specific and targeted sites within the genome. Harnessing the cell’s own natural repair mechanisms, we can cause frameshift mutations leading to gene knockout or provide a template for the cell to repair from leading to targeted insertions.

Image credit: Ding Y, Li H, Chen L-L and Xie K (2016) Recent Advances in Genome Editing Using CRISPR/Cas9. Front. Plant Sci. 7:703. doi: 10.3389/fpls.2016.00703

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