Custom Cell Line Engineering
Have a project perfect for the TUNR Flexible Gene Editing system but need help with the cell line engineering? No problem–Canopy Biosciences offers a comprehensive cell line engineering service based on the CRISPR-Cas9 system. We can integrate TUNR sequences into your target gene in your favorite cell line, as well as take on more standard projects such as gene knockouts, targeted integrations, point mutations, and more.
Cell Lines Available
Canopy has experience editing >80 different cell lines, including popular lines such as HEK-293, HCT-116, NIH 3T3, K562, and HeLa, as well as ES cells and iPSC. Have a unique or highly specialized line? No problem—simply send your line to us and we will characterize the line to see if its amenable to gene editing.
Project Types Available
- TUNR Insertion
- Gene Knockout
- Targeted Deletion
- Codon Change
- Targeted Integration
- Point Mutation
- Gene Tagging
- . . . and more
Knockout projects start at $12,900 and knock-ins at $19,900. Your project may vary based on complexity, fill out our form for a formal project evaluation and quotation.
Get your project started by filling out our form.
ABOUT THE CRISPR-CAS9 SYSTEM
The CRISPR-Cas9 system relies on two components—a targeting domain (sgRNA–orange) and a nuclease domain (Cas9–blue). This system allows for the generation of double-stranded breaks, at highly specific and targeted sites within the genome. Harnessing the cell’s own natural repair mechanisms, we can cause frameshift mutations leading to gene knockout or provide a template for the cell to repair from leading to targeted insertions.
Image credit: Ding Y, Li H, Chen L-L and Xie K (2016) Recent Advances in Genome Editing Using CRISPR/Cas9. Front. Plant Sci. 7:703. doi: 10.3389/fpls.2016.00703