CRISPR Cell Line Service
Have a project perfect for our CRISPR Complete Kit but need a CRISPR cell line service to perform the editing? No problem–Canopy Biosciences offers a comprehensive custom CRISPR cell line service based on the CRISPR-Cas9 system. Just tell us your target gene and the mutation that you want to make, and with our CRISPR cell line service, we’ll make the knockout, knock-in, point mutation, or other modification in your favorite cell line.
Cell Lines Available
Canopy has experience editing >80 different cell lines, including popular lines such as HEK-293, HCT-116, NIH 3T3, K562, and HeLa, as well as ES cells and iPSC. Have a unique or highly specialized line? No problem—simply send your line to us and we will characterize the line to see if its amenable to gene editing.
Project Types Available
- Gene Knockout
- Targeted Deletion
- Codon Change
- Targeted Integration
- Point Mutation
- Gene Tagging
- . . . and more
Knockout projects start at $14,990 and knock-ins at $24,990. Your project may vary based on complexity, fill out our form for a formal project evaluation and quotation.
Get your project started by filling out our form.
ABOUT THE CRISPR-CAS9 SYSTEM
The CRISPR-Cas9 system relies on two components—a targeting domain (sgRNA–orange) and a nuclease domain (Cas9–blue). This system allows for the generation of double-stranded breaks, at highly specific and targeted sites within the genome. Harnessing the cell’s own natural repair mechanisms, we can cause frameshift mutations leading to gene knockout or provide a template for the cell to repair from leading to targeted insertions.
Image credit: Ding Y, Li H, Chen L-L and Xie K (2016) Recent Advances in Genome Editing Using CRISPR/Cas9. Front. Plant Sci. 7:703. doi: 10.3389/fpls.2016.00703