Custom Cell Line Engineering

Have a project perfect for the TUNR Flexible Gene Editing system but need help with the cell line engineering? No problem–Canopy Biosciences offers a comprehensive cell line engineering service based on the CRISPR-Cas9 system. We can integrate TUNR sequences into your target gene in your favorite cell line, as well as take on more standard projects such as gene knockouts, targeted integrations, point mutations, and more.

Step 1: Fill out our Project Evaluation Form.

Canopy’s team of cell line engineers will evaluate the feasibility and scope of your project and provide a formal quotation

Fill out our project evaluation form here.

Step 2: Choose from one of our cell lines or ship us yours.

We’ll evaluate your cell line’s amenability for gene editing.

Step 3: Canopy will custom-design and validate reagents.

All reagents needed to perform your project, including CRISPR guide RNAs and donors for insertion projects, will be custom-designed for your project.

Step 4: Canopy will perform the gene editing.

Canopy will nucleofect your cells with the editing reagents, screen cell pools for modifications, single cell dilute, and screen and identify clones containing your desired mutation.

Step 5: Editing complete!

Following clonal expansion, Canopy will return you your cell line, tailored with your exact modifications.

Fill Out the Project Evaluation Form

Cell Lines Available

Canopy has experience editing >80 different cell lines, including popular lines such as HEK-293, HCT-116, NIH 3T3, K562, and HeLa, as well as ES cells and iPSC. Have a unique or highly specialized line? No problem—simply send your line to us and we will characterize the line to see if its amenable to gene editing.

Project Types Available

TUNR Insertion
Gene Knockout
Targeted Deletion
Codon Change
Targeted Integration
Point Mutation
Gene Tagging
. . . and more

Pricing

Knockout projects start at $12,900 and knock-ins at $19,900. Your project may vary based on complexity, fill out our form for a formal project evaluation and quotation.

Get your project started by filling out our form.

Fill Out the Project Evaluation Form

ABOUT THE CRISPR-CAS9 SYSTEM

The CRISPR-Cas9 system relies on two components—a targeting domain (sgRNA–orange) and a nuclease domain (Cas9–blue). This system allows for the generation of double-stranded breaks, at highly specific and targeted sites within the genome. Harnessing the cell’s own natural repair mechanisms, we can cause frameshift mutations leading to gene knockout or provide a template for the cell to repair from leading to targeted insertions.

Image credit: Ding Y, Li H, Chen L-L and Xie K (2016) Recent Advances in Genome Editing Using CRISPR/Cas9. Front. Plant Sci. 7:703. doi: 10.3389/fpls.2016.00703