Expansion Pack to T Cell Assay Kit: Broad Immune Profiling Assay Kit

8-Plex Add on to T Cell Assay Kit for Human PBMC Samples and ZellScannerONE™
AbKT-1103-10RXN

VistaPlex™ Assay Kits for ChipCytometry offer a ready to use, reliable and customizable solution to fast-track precise spatial multiplexing.

Designed to provide a convenient, flexible, and modular analysis of immune cells, the Expansion Pack to the T Cell Assay Kit provides additional markers to expand the analysis after use of the T Cell Assay Kit. Samples can be stored in the ZellSafe™ Chips (28050606/01-010) for up to 2 years after use of the T Cell Assay Kit, and then assayed with the Expansion Pack to  T Cell Assay Kit to add more biomarkers for analysis. When the two assay kits are combined, they enable quantification of 16 phenotypic biomarkers that define T cell, B cell, Leukocyte, NK cell, NKT cell, Monocyte, Dendritic and Exhaustion cell populations and sub-populations in human PMBC samples and provide the same biomarkers and analysis as the Broad Immune Profiling Assay Kit.

Validation was performed on the ChipCytometry™ platform on 4 blood donors in duplicate, in addition to individual antibody validation. The Expansion Pack to T Cell Assay Kit contains primary antibodies and antibody diluent and is optimized for ease of use and throughput.

Reliable

The ChipCytometry™  platform captures biological variability, while maintaining technical reproducibility. For the Expansion Pack to T Cell Assay Kit, technical replicates were tested on 4 different donors. Fresh PMBC samples were used to confirm intra-assay and inter-assay reproducibility. To ensure high quality data can be obtained on a variety of sample types, the assay kit was successfully tested on cryopreserved samples and samples collected with Cyto-Chex BCT tubes.

Ready to Use and Customizable

The Expansion Pack to T Cell Assay Kit has been optimized to maximize throughput by minimizing the number of cycles required for analysis. One open imaging channel for filter set FS395 in each cycle allows for easy customization. Additional cycles may be added for further customization of your high-plex assay.