Broad Immune Profiling Assay Kit

16-Plex for Human PBMC Samples
AbKT-1002-10RXN

VistaPlex™ Assay Kits for CellScape offer a ready to use, reliable and customizable solution to fast-track precise spatial multiplexing.

Designed to provide a convenient, flexible, and modular analysis of immune cells, the Broad Immune Profiling Assay kit enables quantification of 16 phenotypic biomarkers that define T Cell, B Cell, Leukocyte, NK Cell, NKT Cell, Monocyte, Dendritic and Exhaustion Cell populations and sub-populations in human PMBC samples. Validation was performed on the CellScape platform on 4 blood donors in duplicate, in addition to individual antibody validation. The Broad Immune Profiling Assay Kit contains primary antibodies and antibody diluent and is optimized for ease of use and throughput.

Reliable

The CellScape  platform captures biological variability, while maintaining technical reproducibility. For the Broad Immune Profiling Assay Kit, technical replicates were tested on 4 different donors. Fresh PMBC samples were used to confirm intra-assay and inter-assay reproducibility. To ensure high quality data can be obtained on a variety of sample types, the assay kit was successfully tested on cryopreserved samples and samples collected with Cyto-Chex BCT tubes.

Ready to Use and Customizable

The Broad Immune Profiling Assay Kit has been optimized to maximize throughput by minimizing the number of cycles required for analysis. One open imaging channel for filter set FS395 in each cycle allows for easy customization. Additional cycles may be added for further customization of your high-plex assay.