VistaPlex™ Fresh Frozen Immune Profiling Assay Kit

Foundational Multiplexing for the CellScape™ Platform
AbKT-2001-10RXN

VistaPlex™ Assay Kits for CellScape offer a ready to use, reliable and customizable solution to fast-track precise spatial multiplexing.

Providing a building block for essential spatial discovery of the immune microenvironment, the Fresh Frozen Immune Profiling Assay Kit includes a panel of antibodies curated and optimized by our robust validation workflow. The panel targets key immune biomarkers and can be expanded by pairing with additional VistaPlex assay kits.

Spatially profile the immune landscape with 13 biomarkers and nuclear stain.

HLA-DRCD56CD45RACD8
CD123CD14CD20Nuclear Stain
CD4CD27CD45
PanCytokeratinCD11cCD3

Single-Cell Phenotyping, Spatial Context, and Quantification for at least 23 Immuno-Phenotypes

Rigorous validation includes confirmation of broad tissue suitability, qualitative biomarker specificity, and quantitative reproducibility across technical replicates.

Nucleated cellsLeukocytesEpitheliaNKT cells
NK cellsMemory B cellsT cellsHelper T cells
Cytotoxic T cellsCentral Memory CD4+ cellsNaive CD4+ cellsEffector CD4+ cells
Effector Memory CD4+ cellsCentral Memory CD8+ cellsNaive CD8+ cellsEffector CD8+ cells
Effector Memory CD8+ cellsB cellsNaive B cellsMonocytes
HLA DR+ dendritic cellsPlasmacytoid dendritic cellsMyeloid dendritic cells
The combination of these markers allows researchers to phenotype key tumor and immune cell populations.

Human Fresh Frozen Immune Profiling Assay Kit Documentation

Antibodies for the multiplex panel were validated using three human fresh frozen tissue samples in duplicate to ensure specificity, sensitivity, reproducibility, and multiplex suitability. Download the validation report for experiment details and results.

Store all kit contents at 4°C, protected from light.

Additional materials required: CellScape Cell Chips (PRSM-CHP-TISSUE) and Wash Buffer (PRSM-BUF-WASH).

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