Fibrosis

Human and Mouse

Uncover the mechanisms of disease pathogenesis, identify biomarkers of progression, and develop signatures for therapeutic response with the nCounter Fibrosis Panel. Profile 770 genes across 51 annotated pathways involved in the 4 stages of fibrosis: initiation, inflammation, proliferation, and modification. This gene expression panel combines hundreds of genes involved in the initial tissue damage response, chronic inflammation, proliferation of pro-fibrotic cells, and tissue modification that leads to fibrotic disease of the lungs, heart, liver, kidney, and skin.

BENEFITS
DETAILS & DATA
GENE LIST
PRICING

Benefits

01

Study Pathogenesis and identify biomarkers for fibrotic diseases of the lungs, heart, liver, kidney and skin.

02

Quantify the relative abundance of 14 different immune cell types.

03

Understand signaling cascades from cell stress to inflammation.

04

Identify biomarkers for therapeutic response.

Details & data

Key applications include:

Lung Disease Research

Idiopathic pulmonary fibrosis
Cystic fibrosis

Liver Damage Research

Cirrhosis
Chronic hepatitis C infection
Non-alcoholic steatohepatitis (NASH)
Alcoholic liver disease

Kidney Disease Research

Renal fibrosis

Skin Disease Research

Scleroderma
Nephrogenic fibrosing dermopathy
Scleromyxedma
Scleredema
Eosinophilic fasciitis

Heart Disease Research

Cardiomyopathy
Ischemic heart disease

Gene Coverage Across the Four Stages of Fibrosis

Stage	Description	Pathways	# Human Genes	# Mouse Genes
Initiation	Cell and tissue damage, often specific to an organ or fibrotic disease, initiates a cascade of stress and immune responses.	Autophagy, Cholesterol Metabolism, Cytosolic DNA Sensing, De Novo Lipogenesis, Endotoxin Response, Fatty Acid Metabolism, Gluconeogenesis, Insulin Resistance/Signaling, MAPK Cell Stress, mTOR, Oxidative Stress, PPAR Signaling, Proteotoxic Stress, SASP	369	369
Inflammation	Inflammation is one of many responses to the initial damage, involving multiple immune cell types and signaling pathways. Chronic inflammation drives the proliferation of pro-fibrotic cells and tissue modification.	Adenosine Pathway, Chemokine Signaling, Complement Activation, Cytokine Signaling, Granulocyte Activity, Inflammasome, M1/M2 Activation, MHC Class II Antigen Presentation, Neutrophil Degranulation, NF-kB, Phagocytic Cell Function, Platelet Degranulation, Th1/ Th2/Th17 Differentiation, TLR Signaling, Type I/Type II Interferon	297	297
Proliferation	Differentiation and proliferation of myofibroblasts are driven by upstream inflammation. These cells drive the wound-healing response that results in fibrotic damage.	Cell Cycle, ECM Synthesis, EMT, Focal Adhesion Kinase, Hedgehog Signaling, Hypoxia, Myofibroblast Regulation, Notch, PDGF Signaling, PI3K-Akt, TgfBeta, Wnt	159	161
Modification	Immune and myofibroblast cells contribute to extracellular matrix modification and tissue alterations that are characteristic of fibrotic disease.	Angiogenesis, Apoptosis, Collagen Biosynthesis & Modification, ECM Degradation, Epigenetic Modification, Hippo Pathway, Regulated Necrosis	287	287

Fibrosis Panel Functional Annotations

Functional annotations for different pathways and processes were assigned to the genes in the Fibrosis Panel. The pathways and processes that are included in this panel provide a comprehensive view of the pathogenesis of fibrotic disease.

Annotation	# Human Genes	# Mouse Genes	Annotation	# Human Genes	# Mouse Genes
Adenosine Pathway	51	53	M1 Activation	12	12
Angiogenesis	37	37	M2 Activation	12	12
Autophagy	39	39	MAPK Cell Stress	67	67
Cell Cycle	49	49	MHC Class II Antigen Presentation	16	15
Chemokine Signaling	23	22	mTOR	36	36
Cholesterol Metabolism	26	24	Myofibroblast Regulation	25	25
Collagen Biosynthesis & Modification	27	27	Neutrophil Degranulation	67	73
Complement Activation	22	19	NF-kB	41	40
Cytokine Signaling	60	58	Notch	20	20
De Novo Lipogenesis	20	20	Oxidative Stress	18	18
ECM Degradation	42	44	PDGF Signaling	29	29
ECM Synthesis	38	38	Phagocytic Cell Function	27	27
EMT	88	88	PI3K-Akt	89	89
Endotoxin Response	42	41	Platelet Degranulation	33	34
Epigenetic Modification	24	24	PPAR Signaling	28	33
Fatty Acid Metabolism	55	57	Programmed Cell Death	41	41
Focal Adhesion Kinase	49	49	Proteotoxic Stress	43	43
Glucogenesis	20	19	SASP	15	15
Granulocyte Activity	34	34	TGF-beta	38	38
Hedgehog Signaling	24	24	Th1 Differentiation	13	13
Hippo Pathway	13	13	Th17 Differentiation	24	24
Hypoxia	16	16	Th2 Differentiation	13	13
Inflammasome	32	32	TLR Signaling	63	62
Insulin Resistance	38	38	Type I Interferon	24	31
Insulin Signaling	16	16	Type II Interferon	30	37
Internal Reference Genes	10	10	Wnt	38	38

Immune Cell Profiling

Genes included in the Human Fibrosis Panel provide unique cell profiling data to measure the relative abundance of 14 different human immune cell types . The table below summarizes each cell type represented by gene content in the panel, as qualified through biostatistical approaches and selected literature in the field of immunology.

Cell Type	Associated Human Genes	Cell Type	Associated Human Genes
B cells	BLK, CD19, MS4A1, TNFRSF17, FCRL2, KIAA0125/FAM30A, PNOC, SPIB, TCL1A	Mast Cells	4MS4A2, TPSAB1, CPA3, HDC, TPSB2
CD45	CD45	Neutrophils	CSF3R, S100A12, CEACAM3, FCAR, FCGR3A, FCGR3B, FPR1, SIGLEC5
CD8 T cells	CD8A, CD8B	NK CD56dim cells	IL21R, KIR2DL3, KIR3DL1, KIR3DL2
Cytotoxic cells	CTSW, GNLY, GZMA, GZMB, GZMH, KLRB1, KLRD1, KLRK1, PRF1, NKG7	NK cells	NCR1, XCL2, XCL1
Dendritic cells	CCL13, CD209, HSD11B1	T cells	CD3D, CD3E, CD3G, CD6, SH2D1A, TRAT1
Exhausted CD8	CD244, EOMES, LAG3, PTGER4	Th1 cells	TBX21
Macrophages	CD163, CD68, CD84, MS4A4A	Treg	FOXP3

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Fibrosis

Benefits

Details & data

Key applications include:

Gene Coverage Across the Four Stages of Fibrosis

Fibrosis Panel Functional Annotations

Immune Cell Profiling

Genes in panel

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Fibrosis

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