Wild-type, knockout, and everywhere in between

The TUNR Flexible Gene Editing System from Canopy Biosciences allows you to precisely control the expression of your gene of interest. Our kits are custom-designed for your project and include everything you need to tune gene expression in your favorite cell line.

  • Precisely tune gene expression from 100% all the way down to complete knockout
  • Model ranges of gene expression reflecting diversity in patient populations
  • Control endogenous gene expression–eliminate artifacts from ectopic expression
  • Knockdown essential genes where knockout would result in lethality

The TUNR Flexible Gene Editing System for Endogenous Genes allows you to generate four cell lines or animal models with four different levels of knockdown of your endogenous gene of interest.

The TUNR Targeted Transgenic kit enables you to insert your gene of interest under TUNR control into the AAVS1 locus. AAVS1 is a “safe harbor”—a safe place to integrate transgenes, allowing predictable expression from a locus known not to alter the expression of other genes or have otherwise deleterious effects on the cell.

The TUNR Plasmid-Delivered Transgenes kit allows you to control the expression of a transgene. Simply transfect your cells with one of our four plasmids containing your gene under the control of TUNRs of different strengths.

The secret? PolyA tracks


As recently published in
Nature Communications by Sergej Djuranovic’s lab at Washington University in Saint Louis, insertion of tracks of repeated adenosine bases results in decreased mRNA and protein expression.
TUNR expression levels

 

Shown are wild-type, resulting in normal expression levels, insertion of a short polyA track, resulting in a moderate decrease in expression, and insertion of a long polyA track, resulting in a large decrease in expression. Proteins represented by blue lines.

Adapted from: Arthur, L. L. et al. Rapid generation of hypomorphic mutations. Nat. Commun. 8, 14112 doi: 10.1038/ncomms14112 (2017).

 

 

 

How the TUNR Flexible Gene Editing System works

Introduction of the TUNR polyA track results in reduced gene expression.

The TUNR Flexible Gene Editing System leverages the CRISPR-Cas9 system for targeted integration of a polyA track at a precise
location within the targeted gene.

License

Canopy Biosciences holds the exclusive license for TUNR from Washington University and Johns Hopkins University.

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