A complete service offering for error corrected sequencing.
Increase sensitivity of your NGS experiment 100X to discover what you’ve never been able to measure
Separate the Signal from the Noise
The standard NGS process introduces errors during PCR and the sequencing workflow. These errors result in noise that makes it challenging to differentiate between errors and true variants.
RareSeq eliminates the noise
How it Works
We use a proprietary unique molecular index strategy combined with a custom bioinformatics pipeline to identify ultra-rare variants- down to 1:10,000
Sequencing errors are corrected by aligning reads sharing the same UMI
Ultra-sensitive and Quantitative
Detect and quantitate previously undetectable mutations in heterogeneous DNA with RareSeq. Quantitative to a variant allele frequency (VAF) of 1:10,000, RareSeq is a highly reproducible and sensitive to identify indels and SNVs.
Accuracy and Precision
The error rate of NGS has been reported to be between 1-3%. Ensuring variant detection is accurate, RareSeq is validated by ddPCR demonstrating the high degree of correlation in identified variant allele fractions (VAFs).